.

Evaluation of the infestation with Spotted Wing Drosophila by fruit examination

  • at least 1 times weekly when fruit change colour
  • Samples of 30 fruits randomly picked from within and 30 fruits outside of netting
  • Fruit inspection can be done by visual check of eggs, by expelling larvae using the water method or by emergence of adults
  • Detailed information to the identification of male and female flies, different larval stages, pupa and eggs od Spotted Wing Drosophila on Knowledge Portal Drosophila

Checking eggs and pupae

    • Fruits will be observed for eggs or pupae by magnifying glass or microscope
    • Detailed information to the egg and pupa identification of Spotted Wing Drosophila on Knowledge Portal Drosophila
Sichtbare Eiablage der Kirschessigfliege an Kirschen_C. Augel_LTZ AugustenbergDie zwei typischen Atemfäden eines Kirschessigfliegen-Ei in Kirsche_JKI Dossenheim
Visible oviposition of Spotted Wing Drosophila on cherry (© C. Augel, LTZ Augustenberg)Typical two breathing tubes of Spotted Wing Drosophila egg on cherry (© JKI Dossenheim)
Typische Löcher der Kirschessigfliege in Kirschen_JKI DossenheimTypischer Saftaustritt aus Löchern bei Kirschessigfliegenbefall in Kirschen_JKI Dossenheim
Typical puncture holes caused by oviposition of Spotted Wing Drosophila on cherry (© JKI Dossenheim)Juice discharge through oviposition holes by pressing fruit (© JKI Dossenheim)
Geschlüpfte Kirschessigfliegenpuppe in einer Kirsche_JKI DossenheimDrei geschlüpfte Kirschessigfliegenpuppen in Kirsche_JKI Dossenheim
Empty Spotted Wing Drosophila pupa protruding from cherry fruit (© JKI Dossenheim)Three empty Spotted Wing Drosopila pupae protruding from cherry (© JKI Dossenheim)
Pupa of D. suzukii on cherry_copyright by H. Vogt, JKI DossenheimPupa of D. suzukii_copyright by J. Just, JKI Dossenheim
Pupa of Spotted Wing Drosophila on cherry
(© H. Vogt, JKI Dossenheim) 
Pupa of Spotted Wing Drosophila
(© J. Just, JKI Dossenheim)
Eiablage von D. suzukii an Brombeere_copyright by A. Fried_Landratsamt KarlsruheEiablage an Brombeere_copyright by J. Just, JKI Dossenheim
Oviposition of Spotted Wing Drosophila on blackberry
(© A. Fried, Landratsamt Karlsruhe)
Egg with two typival breathing tube of Spotted Wing Drosophila on blackberry (© J. Just, JKI Dossenheim)
Eiablage an Himbeere_copyright J. Just_JKI DossenheimD. suzukii Ei in Himbeere_copyright J. Just, JKI Dossenheim
Oviposition of Spotted Wing Drosophila on raspberry
(© J. Just, JKI Dossenheim)
Egg of Spotted Wing Drosophila with typical breathing tubes on raspberry (© J. Just, JKI Dossenheim)
Kirschessigfliege bei der Eiablage an einer Himbeere_C. Augel_LTZ AugustenbergKirschessigfliegenlarve am Zapfen einer Himbeere_C. Augel_LTZ Augustenberg
Spotted Wing Drosophila during oviposition on raspberry (© C. Augel, LTZ Augustenberg)Spotted Wing Drosophila larva on raspberry receptacle
(© C. Augel, LTZ Augustenberg)
Eiablage an Heidelbeere_copyright C. Augel, LTZ AugustenbergEiablage an Heidelbeere_copyright C. Augel, LTZ Augustenberg
Visible hole with egg of Spotted Wing Drosophila on blueberry
(© C. Augel, LTZ Augustenberg)
Egg with breathing tubes of Spotted Wing Drosophila on blueberry
(© C. Augel, LTZ Augustenberg)
Sichtbare Löcher durch Kirschessigfliegen-Befall an Heidelbeere_C. Augel_LTZ AugustenbergSichtbare Atemfäden eines Kirschessigfliegen-Ei in Heidelbeere_C. Augel_LTZ Augustenberg
Typical puncture holes caused by oviposition of Spotted Wing Drosophila on blueberries (© C. Augel, LTZ Augustenberg)Two typical breathing tubes of Spotted Wing Drosophila egg on blueberry (© C. Augel, LTZ Augustenberg) 
Geschlüpfte Kirschessigfliegenpuppe in einer Heidelbeere_B. Boehnke_JKI DossenheimUngeschlüpfte Puppe einer Kirschessigfliege von einer Heidelbeere_B. Boehnke_JKI Dossenheim
Empty Spotted Wing Drosophila pupa protruding from blueberry fruit (© B. Boehnke, JKI Dossenheim)Pupa of unhatched Spotted Wing Drosophila from blueberry (© B. Boehnke, JKI Dossenheim)

Checking larvae

    • For exact identification of larval infestation by water method should be used single fruits or samples with a few fruits (e.g. up to 3)
    • Because of possible confusion with other drosophelid larvae should be used fresh fruits without any damages 
    • Hard-shelled fruit like cherries have to be opened, soft fruits like berries can be left intact
    • For detemination of the average number of larvae per fruit, immerse fruits in water   
    • Washing larvae can be done in:  
      • 10 % salty water, at least 1 h (larvae swim partly on the surface of the salty water)
      • tap water, at least 2 h (larvae sink to the bottom)
    • Carefully removing of fruits, counting of expelled larvae by stereo magnifier, recording of number of larvae
    • Detailed information to fruit examination by using salty water method (PDF) Coming soon
    • Detailed information to fruit examination by using salty water method (Video) Coming soon
    • Detailed information to identification of different larval stages of Spotted Wing Drosophila on Knowledge Portal Drosophila
Kontrolle auf D. suzukii an Kirschen mittels Fruchteinzelproben_copyright A. Fried, Landratsamt KarlsruheKontrolle auf D. suzukii an Brombeere mittels Fruchteinzelproben_copyright A. Fried, Landratsamt Karlsruhe
Checking of larvae in cherry fruit individual sample
(© A. Fried, Landratsamt Karlsruhe)
Checking of larvae in blackberry fruit individual sample
(© A. Fried, Landratsamt Karlsruhe)
SWD-larvae on water surface_raspberry_salty water method_B.Boehnke_JKI DossenheimLarven und Himbeeren nach dem Auswaschen der Larven bei starkem Befall im feinmaschigem Sieb_B. Boehnke_JKI Dossenheim
Larvae from three strong infested raspberries on water surface after using salty water method (© B. Boehnke, JKI Dossenheim) Larvae from three strong infested raspberries with raspberries in sieve after using salty water method
(© B. Boehnke, JKI Dossenheim)
Auswaschen von Kirschessigfliegenarven aus großen Heidelbeer-Fruchtproben_C. Augel_LTZ AugustenbergLarven auf der Wasseroberfläche nach dem Auswaschen aus Heidelberen mittels der Salzwassermethode_C. Augel_LTZ Augustenberg
Fruit examination of big blueberry probe by salty water method (© C. Augel, LTZ Augustenberg)Larvae from big blackberry probe on water surface after using salty water method (© C. Augel, LTZ Augustenberg)
Larvenkontrolle in Himbeeren mittels 10% Salzwasser (copyright by Jürgen Just)Larvenkontrolle in Himbeeren in Leitungswasser (copyright by Jürgen Just)
Checking of larvae in raspberries in 10% salty water
(© J. Just, JKI Dossenheim)
Checking of larvae in raspberries in tap water
(© J. Just, JKI Dossenheim)
Drosophila suzukii Larven aus Himbeeren auf der Oberfläche von 10%igem Salzwasser (copyright by Jürgen Just)Auf den Boden abgesunkene Drosophila suzukii Larven aus Himbeeren in Leitungswasser (copyright by Jürgen Just)
Spotted Wing Drosophila larvae from raspberries on the surface of 10% salty water (© J. Just, JKI Dossenheim)Spotted Wing Drosophila larvae from raspberries on the bottom of tap water (© J. Just, JKI Dossenheim)


Checking by hatching method

    • Individual fruit samples were incubated until hatching
    • Detailed information to identification of male and female Spotted Wing Drosophila on Knowledge Portal Drosophila
Fruchtbonitur mittels Schlupf der Fliegen_copyright by B. Boehnke, JKI DossenheimGeschlüpfte Fliegen_copyright by B. Boehnke, JKI Dossenheim
Incubation of individual fruit samples until hatching of adults (© B. Boehnke, JKI Dossenheim)From cherry hatched flies (© B. Boehnke, JKI Dossenheim)
Fruchtbonitur mittels Schlupfkontrolle bei Heidelbeeren_B. Boehnke_JKI DossenheimGeschlüpfte Kirschessigfliegen bei der Fruchtbonitur mittels Schlupfkontrolle_B. Boehnke_JKI Dossenheim
Fruit examination by hatching method on blueberries 
(© B. Boehnke, JKI Dossenheim)
From blueberries hatched Spotted Wing Drosophila
(© B. Boehnke, JKI Dossenheim)

 

 

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