Evaluation of the infestation with Spotted Wing Drosophila by fruit examination
- Fruit examination have to be done at least 1 times weekly from fruit changing colour
- Randomly picked samples of 30 fruits from within and 30 fruits outside of netting
- Fruit inspection can be done by visual checking of eggs, by expelling larvae using the water method or by emergence of adults
- Detailed information to the identification of male and female flies, different larval stages, pupa and eggs of Spotted Wing Drosophila on Knowledge Portal Drosophila
Checking eggs and pupae
- Fruits will be observed for eggs or pupae by magnifying glass or microscope
- Detailed information to the egg and pupa identification of Spotted Wing Drosophila on Knowledge Portal Drosophila
 |  |
| Visible oviposition of Spotted Wing Drosophila on cherry (© C. Augel, LTZ Augustenberg) | Typical two breathing tubes of Spotted Wing Drosophila egg on cherry (© JKI Dossenheim) |
 |  |
| Typical puncture holes caused by oviposition of Spotted Wing Drosophila on cherry (© JKI Dossenheim) | Juice discharge through oviposition holes by pressing fruit (© JKI Dossenheim) |
 |  |
| Empty Spotted Wing Drosophila pupa protruding from cherry fruit (© JKI Dossenheim) | Three empty Spotted Wing Drosopila pupae protruding from cherry (© JKI Dossenheim) |
 |  |
| Pupa of Spotted Wing Drosophila on cherry (© H. Vogt, JKI Dossenheim) | Pupa of Spotted Wing Drosophila (© J. Just, JKI Dossenheim) |
 |  |
| Oviposition of Spotted Wing Drosophila on blackberry (© A. Fried, Landratsamt Karlsruhe) | Egg with two typical breathing tube of Spotted Wing Drosophila on blackberry (© J. Just, JKI Dossenheim) |
 |  |
| Oviposition of Spotted Wing Drosophila on raspberry (© J. Just, JKI Dossenheim) | Egg of Spotted Wing Drosophila with typical breathing tubes on raspberry (© J. Just, JKI Dossenheim) |
 |  |
| Spotted Wing Drosophila during oviposition on raspberry (© C. Augel, LTZ Augustenberg) | Spotted Wing Drosophila larva on raspberry receptacle (© C. Augel, LTZ Augustenberg) |
 |  |
| Visible hole with egg of Spotted Wing Drosophila on blueberry (© C. Augel, LTZ Augustenberg) | Egg of Spotted Wing Drosophila with breathing tubes on blueberry (© C. Augel, LTZ Augustenberg) |
 |  |
| Typical puncture holes caused by oviposition of Spotted Wing Drosophila on blueberries (© C. Augel, LTZ Augustenberg) | Two typical breathing tubes of Spotted Wing Drosophila egg on blueberry (© C. Augel, LTZ Augustenberg) |
 |  |
| Empty Spotted Wing Drosophila pupa protruding from blueberry fruit (© B. Boehnke, JKI Dossenheim) | Pupa of unhatched Spotted Wing Drosophila from blueberry (© B. Boehnke, JKI Dossenheim) |
Checking larvae
- For exact identification of larval infestation by water method should be used single fruits or samples with few fruits (e.g. up to 3)
- Because of possible confusion with other drosophelid larvae should be used fresh fruits without any damages
- Hard-shelled fruit like cherries have to be opened, soft fruits like berries can be left intact
- For detemination of the average number of larvae per fruit, immerse fruits in water
- Washing larvae can be done in:
- 10 % salty water, at least 1 h (larvae swim partly on the surface of the salty water)
- tap water, at least 2 h (larvae sink to the bottom)
- Carefully removing of fruits, counting of expelled larvae by stereo magnifier, recording of number of larvae
- Detailed information to fruit examination by using salty water method (PDF) Coming soon
- Detailed information to fruit examination by using salty water method (Video) Coming soon
- Detailed information to identification of different larval stages of Spotted Wing Drosophila on Knowledge Portal Drosophila
 |  |
| Checking of larvae in cherry fruit individual samples (© A. Fried, Landratsamt Karlsruhe) | Checking of larvae in blackberry fruit individual samples (© A. Fried, Landratsamt Karlsruhe) |
 |  |
| Larvae from three strong infested raspberries on water surface after using salty water method (© B. Boehnke, JKI Dossenheim) | Larvae from three strong infested raspberries with raspberries in sieve after using salty water method (© B. Boehnke, JKI Dossenheim) |
 |  |
| Fruit examination of big blueberry probe by salty water method (© C. Augel, LTZ Augustenberg) | Larvae from big blackberry probe on water surface after using salty water method (© C. Augel, LTZ Augustenberg) |
 |  |
| Checking of larvae in raspberries in 10% salty water (© J. Just, JKI Dossenheim) | Checking of larvae in raspberries in tap water (© J. Just, JKI Dossenheim) |
 |  |
| Spotted Wing Drosophila larvae from raspberries on the surface of 10% salty water (© J. Just, JKI Dossenheim) | Spotted Wing Drosophila larvae from raspberries on the bottom of tap water (© J. Just, JKI Dossenheim) |
Checking by hatching method
- Individual fruit samples were incubated until hatching of flies
- Identification of male and female Spotted Wing Drosophila
- Detailed information to identification of male and female Spotted Wing Drosophila on Knowledge Portal Drosophila
 |  |
| Incubation of individual fruit samples until hatching of adult flies (© B. Boehnke, JKI Dossenheim) | From cherry hatched flies (© B. Boehnke, JKI Dossenheim) |
 |  |
| Fruit examination by hatching method on blueberries (© B. Boehnke, JKI Dossenheim) | From blueberries hatched Spotted Wing Drosophila (© B. Boehnke, JKI Dossenheim) |
This article is already 3 Once seen.