.

Evaluation of the infestation with Spotted Wing Drosophila by fruit examination

  • With yellowing of fruits at least 2 times weekly
  • Samples of 30 fruits randomly picked from within and 30 fruits outside of netting
  • Fruit inspection can be done by visual check of eggs, by expelling larvae using the water method or by emergence of adults

Checking eggs and pupae

  • Fruits will be observed for eggs or pupae by magnifying glass or microscope
Typische Löcher durch Eiablage von D. suzukii an Kirschen_copyright by F. briem, JKI DossenheimEiablage an Kirsche_copyright by F. Briem, JKI Dossenheim
Typical holes by oviposition of D. suzukii in cherry
(© F. Briem, JKI Dossenheim)
Egg of D. suzukii in cherry
(© F. Briem, JKI Dossenheim)
Eiablage von D. suzukii an Brombeere_copyright by A. Fried_Landratsamt KarlsruheEiablage an Brombeere_copyright by J. Just, JKI Dossenheim
Oviposition of D. suzukii in blackberry
(© A. Fried, Landratsamt Karlsruhe)
Egg of D. suzukii in blackberry
(© J. Just, JKI Dossenheim)
Eiablage an Himbeere_copyright J. Just_JKI DossenheimD. suzukii Ei in Himbeere_copyright J. Just, JKI Dossenheim
Oviposition of D. suzukii in raspberry
(© J. Just, JKI Dossenheim)
Egg of D. suzukii in raspberry (© J. Just, JKI Dossenheim)
Eiablage an Heidelbeere_copyright C. Augel, LTZ AugustenbergEiablage an Heidelbeere_copyright C. Augel, LTZ Augustenberg
Oviposition of D. suzukii in blueberry
(© C. Augel, LTZ Augustenberg)
Egg of D. suzukii in blueberry
(© C. Augel, LTZ Augustenberg)
Puppe von D. suzukii an Kirsche_copyright by H. Vogt, JKI DossenheimPuppe von D. suzukii_copyright by J. Just, JKI Dossenheim
Pupa of D. suzukii on cherry
(© H. Vogt, JKI Dossenheim)
Pupa of D. suzukii
(© J. Just, JKI Dossenheim)

Checking larvae

  • Single fruits or samples with a few fruits (e.g. up to 3) should be used when checking larval infestation with the water method
  • Cherries have to be opened, berries can be left intact
  • For detemination of the average number of larvae per fruit, immerse fruits in water    
    • in 10 % salty water at least 1 h (larvae swim partly on the surface of the salty water)
    • in tap water at least 2 h (larvae sink to the bottom)
  • Carefully removing of fruits, counting of expelled larvae by stereo magnifier, record of number of larvae
Kontrolle auf D. suzukii an Kirschen mittels Fruchteinzelproben_copyright A. Fried, Landratsamt KarlsruheKontrolle auf D. suzukii an Brombeere mittels Fruchteinzelproben_copyright A. Fried, Landratsamt Karlsruhe
Checking of larvae in cherry fruit individual sample
(© A. Fried, Landratsamt Karlsruhe)
Checking of larvae in blackberry fruit individual sample
(© A. Fried, Landratsamt Karlsruhe)
Larvenkontrolle in Himbeeren mittels 10% Salzwasser (copyright by Jürgen Just)Larvenkontrolle in Himbeeren in Leitungswasser (copyright by Jürgen Just)
Checking of larvae in raspberries in 10% salty water
(© J. Just, JKI Dossenheim)
Checking of larvae in raspberries in tap water
(© J. Just, JKI Dossenheim)
Drosophila suzukii Larven aus Himbeeren auf der Oberfläche von 10%igem Salzwasser (copyright by Jürgen Just)Auf den Boden abgesunkene Drosophila suzukii Larven aus Himbeeren in Leitungswasser (copyright by Jürgen Just)
Spotted Wing Drosophila larvae from raspberries on the surface of 10% salty water (© J. Just, JKI Dossenheim)Spotted Wing Drosophila larvae from raspberries on the bottom of tap water (© J. Just, JKI Dossenheim)


Checking by adult emergence

  • Individual samples were incubated until hatching
Fruchtbonitur mittels Schlupf der Fliegen_copyright by B. Boehnke, JKI DossenheimGeschlüpfte Fliegen_copyright by B. Boehnke, JKI Dossenheim
Incubation of individual fruit samples until emergence of adults (© B. Boehnke, JKI Dossenheim)Emerged flies (© B. Boehnke, JKI Dossenheim)

 

 

This article is already 2 Once seen.

Powered by Papoo 2012
16970 Vistor